RNA-Seq - Transcriptomics

Sample type:

Plasma, Blood, extracted RNA/DNA

Turn-around

7 working days

Service details

RNA-Seq (RNA sequencing), also called whole transcriptome shotgun sequencing[2] (WTSS), uses next-generation sequencing (NGS) to reveal the presence and quantity of RNA in a biological sample at a given moment.

RNA-Seq is used to analyze the continuously changing cellular transcriptome. Specifically, RNA-Seq facilitates the ability to look at alternative gene spliced transcripts, post-transcriptional modifications, gene fusion, mutations/SNPs and changes in gene expression over time, or differences in gene expression in different groups or treatments.[5] In addition to mRNA transcripts, RNA-Seq can look at different populations of RNA to include total RNA, small RNA, such as miRNA, tRNA, and ribosomal profiling.[6] RNA-Seq can also be used to determine exon/intron boundaries and verify or amend previously annotated 5' and 3' gene boundaries. Recent advances in RNA-seq include single cell sequencing and in situ sequencing of fixed tissue. Source (Wikipedia).

We have access to NexSeq 500 and HiSeq to RNA-Seq data generation. We have also a number of validated RNA-Seq bioinformatics pipelines.

Category

DNA Sequencing

Sub-category

Illumina MiSeq

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Please note that academic price involves subsidizing the production of data and KRISP members producing (max. two individuals) and/or analyzing data are normally co-authors to manuscripts. The commercial price include all costs and no-authorship or collaborationn is needed. Price can vary due to exchange rates, price last estimated at - 2019-03-10