Title: Dried Blood Spot HIV-1 RNA Quantification Using Open Real-Time Systems in South Africa and Burkina Faso
Authors: Viljoen J, Gampini S, Danaviah S, Valea D, Pillay S, Kania D, Meda N, Newell ML, Van de Perre P, Rouet F; for the World Health Organization/ANRS 1289 Kesho Bora Study Group.
Journal: Acquir Immune Defic Syndr,55(3):290-8 (2010)
There is an urgent need to assess the accuracy/feasibility
of using dried blood spots (DBS) for monitoring of HIV-1 viral load
in resource-limited settings. A total of 892 DBS from HIV-1?positive
pregnant women and their neonates enrolled in the Kesho Bora
prevention of mother-to-child transmission trial conducted in Durban
(South Africa) and Bobo-Dioulasso (Burkina Faso) between May
2005 and July 2008 were tested for HIV-1 RNA. The combination
Nuclisens extraction method (BioMetrieux)/Generic HIV Viral Load
assay (Biocentric) was performed using one DBS (in Durban) versus
2 DBS (in Bobo-Dioulasso) on 2 distinct open real-time polymerase
chain reaction instruments.
DBS HIV-1 RNA results were compared
with plasma HIV-1 RNA and HIV serology results used as the
gold standards. The limits of detection of assays on DBS were 3100
and 1550 copies per milliliter in Durban and Bobo-Dioulasso,
respectively. DBS HIV-1 RNA values correlated significantly with
plasma levels (n = 327; R = 0.7351) and were uniformly distributed
according to duration of DBS storage at 220C (median duration,
280 days). For early infant diagnosis, the sensitivity and specificity
were 100% (95% confidence interval: 97.2 to 100.0 and 96.5 to
HIV-1 viral load kinetics in DNase-pretreated
DBS were similar to those obtained in plasma specimens among 13
patients receiving antiretroviral treatment. HIV-1 RNA findings from
serial infant DBS collected prospectively (n = 164) showed 100%
concordance with HIV serology at 18 months of life. Our findings
strongly advocate the implementation of DBS HIV-1 RNA testing in
remote areas from low-income and middle-income countries.
Citation: Viljoen J, Gampini S, Danaviah S, Valea D, Pillay S, Kania D, Meda N, Newell ML, Van de Perre P, Rouet F; for the World Health Organization/ANRS 1289 Kesho Bora Study Group. Dried Blood Spot HIV-1 RNA Quantification Using Open Real-Time Systems in South Africa and Burkina Faso Acquir Immune Defic Syndr,55(3):290-8 (2010).