HIV-1 genotyping service in Africa: rapid, reliable, and cost-effective. KRISP and Thermo Fisher Scientific 2019

SPOTLIGHT


Science and PLoS NTD


Promotion Sanger


KRISP News

KRISP NEWS

HIV-1 genotyping service in Africa: rapid, reliable, and cost-effective

KRISP, Applied Biosystems and Thermo Fisher Scientific collaborate to bring Africa one step closer to realizing the 90-90-90 target by introducing a rapid, reliable, and cost-effective HIV-1 genotyping service in Africa. As part of the introduction, we are running a promotion of ZAR800 (US$ 55) per genotyping test (Jul-Dec 2019). Test can be done in plasma or DBS samples, turn-around time is seven working days.


Bioinformatics tools: Computational methods for the identification of Dengue, Zika, Chikungunya and Yellow Fever Virus species and genotypes

We recently produced open access phylogenetic typing tools to classify Arboviruses, including Dengue, Zika, Chikungunya and Yellow Fever Virus. These tools are been widely used by the scientific community and were published in Science (2018) and PLoS NTD (2019).


SPARK GLOBAL Program & Its value Proposition in Africa - Durban SPARK Innovation Breakfast, 27 June 2019

Durban SPARK Innovation Breakfast presentation by Professor Michael Wallach, KRISP Director SPARK Sydney and Regional Director of SPARK Oceania, University of Technology Sydney (UTS), Thursday, 27 June 2019(7:30am - 8:30), K-RITH building, Nelson R Mandela School of Medicine, UKZN, Durban, South Africa. All welcome!


Capillary Electrophoresis (i.e. Sanger Sequencing) Workshop II, Durban, South Africa, 27-28 June 2019

UKZN African Health Flagship, KRISP, DIPLOMICS & Thermo Education Program on Capillary Electrophoresis (i.e. Sanger Sequencing. . Thermo-KRISP Lab, Nelson Mandela School of Medicine, UKZN, Durban, South Africa, 27-28 June 2019. Training is open to University Post-Graduate Students & Technical Staff of Commercial and Academic laboratories. Scholarships available to previously disadvantaged South African learners.


Capillary Electrophoresis (i.e. Sanger Sequencing) Training Workshop, Durban, South Africa, 24-25 June 2019

UKZN African Health Flagship, KRISP, DIPLOMICS & Thermo Education Program on Capillary Electrophoresis (i.e. Sanger Sequencing) . Thermo-KRISP Lab, Nelson Mandela School of Medicine, UKZN, Durban, South Africa, 24-25 June 2019. Training is open to University Post-Graduate Students & Technical Staff of Commercial and Academic laboratories. Scholarships available to previously disadvantaged South African learners.


STEM Education: Training II – Quantitative PCR (qPCR), KRISP and ThermoScientific Workshop, Durban, South Africa, 21 June 2019

Science Technology Engineering and Maths (STEM) training II on quantitative PCR (qPCR). Thermo-KRISP Lab, Nelson Mandela School of Medicine, UKZN, Durban, South Africa, 21 June 2019. Training is open to post-graduate students and for technical staff of commercial and academic laboratories.


STEM Education: Training I – Quantitative PCR (qPCR), KRISP and ThermoScientific Workshop, Durban, South Africa, 20 June 2019

Science Technology Engineering and Maths (STEM) training II on quantitative PCR (qPCR). Thermo-KRISP Lab, Nelson Mandela School of Medicine, UKZN, Durban, South Africa, 20 June 2019. Training is open to post-graduate students and for technical staff of commercial and academic laboratories.


KRISP Newsletter May/June 2019: KRISP participation in BIO 2019, the largest biotechnology innovation convention in the world

This month, we focused our newsletter on KRISP participation in the largest biotechnology innovation convention in the world, BIO2019. KRISP is part of the official South African delegation and is profiled as an inspiring organization for economic development in South Africa. In the newsletter, we also cover our high-throughput Sanger and Illumina sequencing services, growing bioinformatics team and KRISP & Thermo Fisher Scientific training program in Africa.


UKZN secures grant for joint study into mental health

Scientists from UKZN’s College of Health Sciences and KRISP have received a R12,8 million grant from the South African Medical Research Council and the UK Medical Research Council/Newton Fund for a UK-South Africa joint initiative on mental health.


BIO 2019: KRISP is part of the official South Africa Delegation at BIO 2019

KRISP takes part of the South African delegation attending the Bio International Convention. In addition, the delegation will include senior representatives of the DST, SAMRC, TIA, CPGR and DIPLOMICS. Together, we will showcase South Africa in the BIO 2019 as one of the most sophisticated, diverse and dynamic emerging market that is open to investment.


KRISP PAPERS

Circulation of chikungunya virus East/Central/South African lineage in Rio de Janeiro, Brazil.
Xavier J, Giovanetti M, Fonseca V, Thézé J, Gräf T, Fabri A, Goes de Jesus J, Lima de Mendonça MC, Damasceno Dos Santos Rodrigues C, Mares-Guia MA, Cardoso Dos Santos C, Fraga de Oliveira Tosta S, Candido D, Ribeiro Nogueira RM, Luiz de Abreu A, Kleber Oliveira W, Campelo de Albuquerque CF, Chieppe A, de Oliveira T, Brasil P, Calvet G, Carvalho Sequeira P, Rodrigues Faria N, Bispo de Filippis AM, Alcantara LCJ, PLoS One (2019), 14(6):e0217871:.


A computational method for the identification of Dengue, Zika and Chikungunya virus species and genotypes.
Fonseca V, Libin PJK, Theys K, Faria NR, Nunes MRT, Restovic MI, Freire M, Giovanetti M, Cuypers L, Nowé A, Abecasis A, Deforche K, Santiago GA, Siqueira IC, San EJ, Machado KCB, Azevedo V, Filippis AMB, Cunha RVD, Pybus OG, Vandamme AM, Alcantara LCJ, de Oliveira T, PLoS Negl Trop Dis (2019), 13(5):e0007231: doi: 10.1371/journal.pntd.0007231.


Inferring HIV-1 transmission networks and sources of epidemic spread in Africa with deep-sequence phylogenetic analysis.
Ratmann O, Grabowski KM, Hall M, Golubchik T, Wymant C, Abeler-Dorner L, Bonsall D, Hoppe A, Leigh Brown A, de Oliveira T, Gall A, Kellam P, Pillay D, Kagaayi J, Kigozi G, Quinn TC, Wawer MJ, Laeyendecker O, Serwadda D, Gray RH, Fraser C, PANGEA Consortium and Rakai Health Sciences Program , Nature Communications (2019), 10:1411:https://doi.org/10.1038/s41467-019-09139-4.


Trends in Pretreatment HIV-1 Drug Resistance in Antiretroviral Therapy-naive Adults in South Africa, 2000–2016: A Pooled Sequence Analysis.
Chimukangara B, Lessells RJ, Rhee S-Y, Giandhari J, Kharsany ABM, Naidoo K, Lewis L, Cawood C, Khanyile D, Ayalew KA, Diallo K, Samuel R, Hunt G, Vandormael A, Stray-Pedersen B, Gordon M, Makadzange T, Kiepiela P, Ramjee G, Ledwaba J, Kalimashe M, Morris L, Parikh UM, Mellors JW, Shafer RW, Katzenstein D, Moodley P, Gupta RK, Pillay D, Karim SSA, de Oliveira T, EClinicalMedicine (Lancet) (2019), DOI:https://doi.org/10.1016/j.eclinm.2019.03.006:.


Major depression and household food insecurity among individuals with multidrug-resistant tuberculosis (MDR-TB) in South Africa..
Tomita A, Ramlall S, Naidu T, Mthembu SS, Padayatchi N, Burns JK, Soc Psychiatry Psychiatr Epidemiol. (2019), doi: 10.1007/s00127-019-01669-y:.


KRISP VIDEOS

Human Genome, S5Ion Sequencing & Women in Science in Africa
By: Maryam Fish

KRISP TEAM

KRISP BLOGS


Nicolette Crozier, Benjamin Chimukangara, Veron Ramsuran

Meeting of the Swiss, Swedes and South Africans minds


KRISP TOOLS

2019-02-12
Version: 3


REGA HIV Subtyping Tool V3 - Belgium Mirror

Phylogenetic tool to identify the HIV-1 subtypes and recombinants. Query sequences are analysed for recombination using bootscanning methods. The version 3 contains new CRFs (CRF01_AE to CRF47_BF).


2018-12-05
Version:


Booster: a new bootstrap method for construction of large trees

A new version of the phylogenetic bootstrap in which the presence of inferred branches in replications is measured using a gradual 'transfer' distance rather than the binary presence or absence index used in Felsenstein's original version. The resulting supports are higher and do not induce falsely supported branches.


2016-08-21
Version: 3


REGA HIV Subtyping Tool V3 - South African mirror

This tool is designed to use phylogenetic methods in order to identify the HIV-1 subtype of a specific sequence. The sequence is analysed for recombination using bootscanning methods. The version 3 contains new CRFs (CRF01_AE to CRF47_BF).


2016-08-20
Version: 1


ArboTyping: Zika Typing Tool

This is the first version of the Zika typing tool, which uses phylogenetic analysis to identify the species and genotype of the virus.


2016-08-20
Version: 1


Genome Detective

This is the first version of our Arbovirus typing tool for Chikungunya, Dengue, Yellow Fever and Zika


2016-08-20
Version: 1


ArboTyping: Chikungunya Typing Tool

This is the first version of the Chikungunya typing tool, which uses phylogenetic analysis to identify the species and genotype of the virus.