KRISP OMICS SERVICES: Cutting Edge Infrastructure for 
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SPOTLIGHT


Science and PLoS NTD


Promotion Sanger


KRISP News

KRISP NEWS

Bioinformatics tools: Computational methods for the identification of Dengue, Zika, Chikungunya and Yellow Fever Virus species and genotypes

We recently produced open access phylogenetic typing tools to classify Arboviruses, including Dengue, Zika, Chikungunya and Yellow Fever Virus. These tools are been widely used by the scientific community and were published in Science (2018) and PLoS NTD (2019).


DNA Sequencing Fragment Analysis (i.e Microsatellite) Workshop, Durban, South Africa, 27-28 June 2019

UKZN African Health Flagship, KRISP, DIPLOMICS & Thermo Education Program on DNA Sequencing Fragment Analysis Workshop . Thermo-KRISP Lab, Nelson Mandela School of Medicine, UKZN, Durban, South Africa, 27-28 June 2019. Training is open to University Post-Graduate Students & Technical Staff of Commercial and Academic laboratories. Scholarships available to previously disadvantaged South African learners.


Capillary Electrophoresis (i.e. Sanger Sequencing) Training Workshop, Durban, South Africa, 24-25 June 2019

UKZN African Health Flagship, KRISP, DIPLOMICS & Thermo Education Program on Capillary Electrophoresis (i.e. Sanger Sequencing) . Thermo-KRISP Lab, Nelson Mandela School of Medicine, UKZN, Durban, South Africa, 24-25 June 2019. Training is open to University Post-Graduate Students & Technical Staff of Commercial and Academic laboratories. Scholarships available to previously disadvantaged South African learners.


STEM Education: Training II – Quantitative PCR (qPCR), KRISP and ThermoScientific Workshop, Durban, South Africa, 21 June 2019

Science Technology Engineering and Maths (STEM) training II on quantitative PCR (qPCR). Thermo-KRISP Lab, Nelson Mandela School of Medicine, UKZN, Durban, South Africa, 21 June 2019. Training is open to post-graduate students and for technical staff of commercial and academic laboratories.


STEM Education: Training I – Quantitative PCR (qPCR), KRISP and ThermoScientific Workshop, Durban, South Africa, 20 June 2019

Science Technology Engineering and Maths (STEM) training II on quantitative PCR (qPCR). Thermo-KRISP Lab, Nelson Mandela School of Medicine, UKZN, Durban, South Africa, 20 June 2019. Training is open to post-graduate students and for technical staff of commercial and academic laboratories.


Promotion Sanger Sequencing at KRISP - 1 Jun to 1 Dec 2019

KRISP is running a Sanger Sequencing promotion to celebrate the launch of two new Sanger Sequencers (ABI 3730xl and 3500). Unbelievable prices for the first 6 months. Capillary electrophoresis only – R 25 (per sample well). Sequencing of PCR purified amplicons – R 45 (per primer). Sequencing of PCR unpurified amplicons – R 75 (per primer).


KRISP Newsletter Apr/May 2019: Genomics, bioinformatics and artificial intelligence technologies at KRISP and ThermoFisher Workshops

This month, we focused our newsletter on Genomics, bioinformatics and artificial intelligence at KRISP. This month newsletter also highlights our travel to the Wellcome Trust Sanger Institute and a prestigious Royal Society Award to KRISP. We also cover our recent publication on TB and mental health in South Africa and four new training workshops organized with ThermoFisher Scientific.


KRISP Talks: Funding Opportunities: FLAIR Fellowship & SAMRC-NIH Calls 2019

UKZN African Health Flagship Grants Support Unit discuss about new funding opportunities: AAS and Royal Society FLAIR Fellowship & SAMRC-NIH Calls 2019. Wednesday, 24 April 2019 (1:00pm - 2:00pm), K-RITH building, Nelson R Mandela School of Medicine, UKZN, Durban, South Africa. All welcome!


KRISP at IndabaX Deep Learning and Artificial Intelligence Conference, South Africa 2019

The IndabaX is a means with which to experiment with the ways in which we strengthen African machine learning, artificial intelligence and its local communities. KRISP is a sponsor of the Deep Learning IndabaX 2019, 14 to 17 April, South Africa.


SAFM Interview Story Telling for Health Research following our BMJ Medical Humanities 2019 paper

Radio interview by Dr. Astrid Treffry-Goatley about the use of story telling of our BMJ Medical Humanities 2019 paper entitled: Community engagement with HIV drug adherence in rural South Africa: a transdisciplinary approach.


KRISP PAPERS

A computational method for the identification of Dengue, Zika and Chikungunya virus species and genotypes.
Fonseca V, Libin PJK, Theys K, Faria NR, Nunes MRT, Restovic MI, Freire M, Giovanetti M, Cuypers L, Nowé A, Abecasis A, Deforche K, Santiago GA, Siqueira IC, San EJ, Machado KCB, Azevedo V, Filippis AMB, Cunha RVD, Pybus OG, Vandamme AM, Alcantara LCJ, de Oliveira T, PLoS Negl Trop Dis (2019), 13(5):e0007231: doi: 10.1371/journal.pntd.0007231.


Inferring HIV-1 transmission networks and sources of epidemic spread in Africa with deep-sequence phylogenetic analysis.
Ratmann O, Grabowski KM, Hall M, Golubchik T, Wymant C, Abeler-Dorner L, Bonsall D, Hoppe A, Leigh Brown A, de Oliveira T, Gall A, Kellam P, Pillay D, Kagaayi J, Kigozi G, Quinn TC, Wawer MJ, Laeyendecker O, Serwadda D, Gray RH, Fraser C, PANGEA Consortium and Rakai Health Sciences Program , Nature Communications (2019), 10:1411:https://doi.org/10.1038/s41467-019-09139-4.


Trends in Pretreatment HIV-1 Drug Resistance in Antiretroviral Therapy-naive Adults in South Africa, 2000–2016: A Pooled Sequence Analysis.
Chimukangara B, Lessells RJ, Rhee S-Y, Giandhari J, Kharsany ABM, Naidoo K, Lewis L, Cawood C, Khanyile D, Ayalew KA, Diallo K, Samuel R, Hunt G, Vandormael A, Stray-Pedersen B, Gordon M, Makadzange T, Kiepiela P, Ramjee G, Ledwaba J, Kalimashe M, Morris L, Parikh UM, Mellors JW, Shafer RW, Katzenstein D, Moodley P, Gupta RK, Pillay D, Karim SSA, de Oliveira T, EClinicalMedicine (Lancet) (2019), DOI:https://doi.org/10.1016/j.eclinm.2019.03.006:.


Major depression and household food insecurity among individuals with multidrug-resistant tuberculosis (MDR-TB) in South Africa..
Tomita A, Ramlall S, Naidu T, Mthembu SS, Padayatchi N, Burns JK, Soc Psychiatry Psychiatr Epidemiol. (2019), doi: 10.1007/s00127-019-01669-y:.


KRISP VIDEOS

Human Genome, S5Ion Sequencing & Women in Science in Africa
By: Maryam Fish

KRISP TEAM

KRISP BLOGS


Nicolette Crozier, Benjamin Chimukangara, Veron Ramsuran

Meeting of the Swiss, Swedes and South Africans minds


KRISP TOOLS

2019-02-12
Version: 3


REGA HIV Subtyping Tool V3 - Belgium Mirror

Phylogenetic tool to identify the HIV-1 subtypes and recombinants. Query sequences are analysed for recombination using bootscanning methods. The version 3 contains new CRFs (CRF01_AE to CRF47_BF).


2018-12-05
Version:


Booster: a new bootstrap method for construction of large trees

A new version of the phylogenetic bootstrap in which the presence of inferred branches in replications is measured using a gradual 'transfer' distance rather than the binary presence or absence index used in Felsenstein's original version. The resulting supports are higher and do not induce falsely supported branches.


2016-08-21
Version: 3


REGA HIV Subtyping Tool V3 - South African mirror

This tool is designed to use phylogenetic methods in order to identify the HIV-1 subtype of a specific sequence. The sequence is analysed for recombination using bootscanning methods. The version 3 contains new CRFs (CRF01_AE to CRF47_BF).


2016-08-20
Version: 1


ArboTyping: Zika Typing Tool

This is the first version of the Zika typing tool, which uses phylogenetic analysis to identify the species and genotype of the virus.


2016-08-20
Version: 1


Genome Detective

This is the first version of our Arbovirus typing tool for Chikungunya, Dengue, Yellow Fever and Zika


2016-08-20
Version: 1


ArboTyping: Chikungunya Typing Tool

This is the first version of the Chikungunya typing tool, which uses phylogenetic analysis to identify the species and genotype of the virus.