KRISP, Applied Biosystems and Thermo Fisher Scientific collaborate to bring Africa one step closer to realizing the 90-90-90 target by introducing a rapid, reliable, and cost-effective HIV-1 genotyping service in Africa. As part of the introduction, we are running a promotion of ZAR800 (US$ 55) per genotyping test (Jul-Dec 2019). Test can be done in plasma or DBS samples, turn-around time is seven working days.
We recently produced open access phylogenetic typing tools to classify Arboviruses, including Dengue, Zika, Chikungunya and Yellow Fever Virus. These tools are been widely used by the scientific community and were published in Science (2018) and PLoS NTD (2019).
Durban SPARK Innovation Breakfast presentation by Professor Michael Wallach, KRISP Director SPARK Sydney and Regional Director of SPARK Oceania, University of Technology Sydney (UTS), Thursday, 27 June 2019(7:30am - 8:30), K-RITH building, Nelson R Mandela School of Medicine, UKZN, Durban, South Africa. All welcome!
UKZN African Health Flagship, KRISP, DIPLOMICS & Thermo Education Program on Capillary Electrophoresis (i.e. Sanger Sequencing. . Thermo-KRISP Lab, Nelson Mandela School of Medicine, UKZN, Durban, South Africa, 27-28 June 2019. Training is open to University Post-Graduate Students & Technical Staff of Commercial and Academic laboratories. Scholarships available to previously disadvantaged South African learners.
UKZN African Health Flagship, KRISP, DIPLOMICS & Thermo Education Program on Capillary Electrophoresis (i.e. Sanger Sequencing) . Thermo-KRISP Lab, Nelson Mandela School of Medicine, UKZN, Durban, South Africa, 24-25 June 2019. Training is open to University Post-Graduate Students & Technical Staff of Commercial and Academic laboratories. Scholarships available to previously disadvantaged South African learners.
Science Technology Engineering and Maths (STEM) training II on quantitative PCR (qPCR). Thermo-KRISP Lab, Nelson Mandela School of Medicine, UKZN, Durban, South Africa, 21 June 2019. Training is open to post-graduate students and for technical staff of commercial and academic laboratories.
Science Technology Engineering and Maths (STEM) training II on quantitative PCR (qPCR). Thermo-KRISP Lab, Nelson Mandela School of Medicine, UKZN, Durban, South Africa, 20 June 2019. Training is open to post-graduate students and for technical staff of commercial and academic laboratories.
This month, we focused our newsletter on KRISP participation in the largest biotechnology innovation convention in the world, BIO2019. KRISP is part of the official South African delegation and is profiled as an inspiring organization for economic development in South Africa. In the newsletter, we also cover our high-throughput Sanger and Illumina sequencing services, growing bioinformatics team and KRISP & Thermo Fisher Scientific training program in Africa.
Scientists from UKZN’s College of Health Sciences and KRISP have received a R12,8 million grant from the South African Medical Research Council and the UK Medical Research Council/Newton Fund for a UK-South Africa joint initiative on mental health.
KRISP takes part of the South African delegation attending the Bio International Convention. In addition, the delegation will include senior representatives of the DST, SAMRC, TIA, CPGR and DIPLOMICS. Together, we will showcase South Africa in the BIO 2019 as one of the most sophisticated, diverse and dynamic emerging market that is open to investment.
Circulation of chikungunya virus East/Central/South African lineage in Rio de Janeiro, Brazil.
Xavier J, Giovanetti M, Fonseca V, Thézé J, Gräf T, Fabri A, Goes de Jesus J, Lima de Mendonça MC, Damasceno Dos Santos Rodrigues C, Mares-Guia MA, Cardoso Dos Santos C, Fraga de Oliveira Tosta S, Candido D, Ribeiro Nogueira RM, Luiz de Abreu A, Kleber Oliveira W, Campelo de Albuquerque CF, Chieppe A, de Oliveira T, Brasil P, Calvet G, Carvalho Sequeira P, Rodrigues Faria N, Bispo de Filippis AM, Alcantara LCJ, PLoS One (2019), 14(6):e0217871:.
A computational method for the identification of Dengue, Zika and Chikungunya virus species and genotypes.
Fonseca V, Libin PJK, Theys K, Faria NR, Nunes MRT, Restovic MI, Freire M, Giovanetti M, Cuypers L, Nowé A, Abecasis A, Deforche K, Santiago GA, Siqueira IC, San EJ, Machado KCB, Azevedo V, Filippis AMB, Cunha RVD, Pybus OG, Vandamme AM, Alcantara LCJ, de Oliveira T, PLoS Negl Trop Dis (2019), 13(5):e0007231: doi: 10.1371/journal.pntd.0007231.
Neurocognitive Impairment Risk Among Individuals With Multiple Drug-Resistant Tuberculosis and Human Immunodeficiency Virus Coinfection: Implications for Systematic Linkage to and Retention of Care in Tuberculosis/Human Immunodeficiency Virus Treatment..
Tomita A, Ramlall S, Naidu T, Mthembu SS, Padayatchi N, Burns JK, J Nerv Ment Dis (2019), 207(4):307-310.
Inferring HIV-1 transmission networks and sources of epidemic spread in Africa with deep-sequence phylogenetic analysis.
Ratmann O, Grabowski KM, Hall M, Golubchik T, Wymant C, Abeler-Dorner L, Bonsall D, Hoppe A, Leigh Brown A, de Oliveira T, Gall A, Kellam P, Pillay D, Kagaayi J, Kigozi G, Quinn TC, Wawer MJ, Laeyendecker O, Serwadda D, Gray RH, Fraser C, PANGEA Consortium and Rakai Health Sciences Program , Nature Communications (2019), 10:1411:https://doi.org/10.1038/s41467-019-09139-4.
Trends in Pretreatment HIV-1 Drug Resistance in Antiretroviral Therapy-naive Adults in South Africa, 2000–2016: A Pooled Sequence Analysis.
Chimukangara B, Lessells RJ, Rhee S-Y, Giandhari J, Kharsany ABM, Naidoo K, Lewis L, Cawood C, Khanyile D, Ayalew KA, Diallo K, Samuel R, Hunt G, Vandormael A, Stray-Pedersen B, Gordon M, Makadzange T, Kiepiela P, Ramjee G, Ledwaba J, Kalimashe M, Morris L, Parikh UM, Mellors JW, Shafer RW, Katzenstein D, Moodley P, Gupta RK, Pillay D, Karim SSA, de Oliveira T, EClinicalMedicine (Lancet) (2019), DOI:https://doi.org/10.1016/j.eclinm.2019.03.006:.
Effects of Migration on Risky Sexual Behavior and HIV Acquisition in South Africa: A Systematic Review and Meta-analysis, 2000-2017..
Dzomba A, Tomita A, Govender K, Tanser F, AIDS Behav. (2019), doi: 10.1007/s10461-018-2367-z:.
Major depression and household food insecurity among individuals with multidrug-resistant tuberculosis (MDR-TB) in South Africa..
Tomita A, Ramlall S, Naidu T, Mthembu SS, Padayatchi N, Burns JK, Soc Psychiatry Psychiatr Epidemiol. (2019), doi: 10.1007/s00127-019-01669-y:.
Human Genome, S5Ion Sequencing & Women in Science in Africa
By: Maryam Fish
Phylogenetic tool to identify the HIV-1 subtypes and recombinants. Query sequences are analysed for recombination using bootscanning methods. The version 3 contains new CRFs (CRF01_AE to CRF47_BF).
A new version of the phylogenetic bootstrap in which the presence of inferred branches in replications is measured using a gradual 'transfer' distance rather than the binary presence or absence index used in Felsenstein's original version. The resulting supports are higher and do not induce falsely supported branches.
This tool is designed to use phylogenetic methods in order to identify the HIV-1 subtype of a specific sequence. The sequence is analysed for recombination using bootscanning methods. The version 3 contains new CRFs (CRF01_AE to CRF47_BF).
This is the first version of the Zika typing tool, which uses phylogenetic analysis to identify the species and genotype of the virus.
This is the first version of our Arbovirus typing tool for Chikungunya, Dengue, Yellow Fever and Zika
This is the first version of the Chikungunya typing tool, which uses phylogenetic analysis to identify the species and genotype of the virus.